Journal: BMC Urology

Article Title: Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway

doi: 10.1186/s12894-020-00731-1

Figure Lengend Snippet: MTA1 regulated the expression of metastasis-related factors via the NFκB pathway. A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, siNC, and si-MTA1. a Protein levels of MTA1; cells were collected for WB with MTA1 and GAPDH antibodies. The bands of over-expressed MTA1 were from Additional file : Fig. 2A and down-expressed MTA1 were from Additional file : Fig. 2B with arrow. b Cells were lysed and used in qRT-PCR assays to measure the E-cadherin mRNA expression. Statistical analysis was performed using Student’s t test. b , c A498 cells were transfected with pcDNA3.1-Flag and Flag-MTA1. After 36 h, the Flag-MTA1 group was treated with 10 nM PDTC for 12 h. Then cells were harvested for qRT-PCR evaluation to measure the mRNA expression of MMP2 ( b ) and MMP9 ( c ). Statistical analysis was performed using ANOVA. d A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, siNC, and si-MTA1. After 48 h, cells were subjected to western blotting analysis using antibodies targeting p-p65, p65 and ACTB. The bands of p-p65, p65 and ACTB were from Additional file : Fig. 3. e, f Image J was used to calculate the gray scanned bands of p-p65 ( b ) and p65 (C). * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001; NS not significant

Article Snippet: The human kidney cancer cell line A498 was purchased from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Expressing, Transfection, Quantitative RT-PCR, Western Blot

Journal: BMC Urology

Article Title: Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway

doi: 10.1186/s12894-020-00731-1

Figure Lengend Snippet: MTA1 promotes A498 cell migration. A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, siNC, and si-MTA1. After 24 h, cells in the four conditions were subjected to the wound healing assay. Macrographs were taken under ×100 magnification

Article Snippet: The human kidney cancer cell line A498 was purchased from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Migration, Transfection, Wound Healing Assay

Journal: BMC Urology

Article Title: Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway

doi: 10.1186/s12894-020-00731-1

Figure Lengend Snippet: MTA1 enhances the invasion of A498 cells. a A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, siNC, and si-MTA1. After 24 h, the transwell cell invasion assay using A498 cells was performed, and macrographs were taken under ×40 magnification. Scale bar: 50 µm. b Image J was used to count the transmigrated cells and Student’s t test to analyze differences. ** p < 0.01; *** p < 0.001

Article Snippet: The human kidney cancer cell line A498 was purchased from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Transfection, Invasion Assay

Journal: BMC Urology

Article Title: Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway

doi: 10.1186/s12894-020-00731-1

Figure Lengend Snippet: MTA1 regulated the migration and invasion of RCC cells via NF-κB. A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, and Flag-MTA1 + PDTC (10 nM). a In the wound healing assay, MTA1 improved the migration of RCC cells, while the addition of the inhibitor PDTC blocked the effect of MTA1 on migration. Scale bar: 100×. b In the transwell assay, MTA1 markedly induced invasion of A498 cells, but compared to the MTA1 group, the invasion of MTA1 + PDTC A498 cells was lower. ** p < 0.01; *** p < 0.001; Scale bar: ×40

Article Snippet: The human kidney cancer cell line A498 was purchased from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Migration, Transfection, Wound Healing Assay, Transwell Assay

Journal: BMC Urology

Article Title: Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway

doi: 10.1186/s12894-020-00731-1

Figure Lengend Snippet: The expression of MTA1 is up-regulated in RCCs. a Immunohistochemistry was used to characterize the expression of MTA1 in RCC and adjacent tissues. MTA1 was highly expressed in ccRCCs, compared to very weak staining in adjacent tissue. b Image Pro Plus was used for the statistical analysis of the positive signal (IOD) of MTA1 expression in ccRCCs and the adjacent tissue. The expression of MTA1 was significantly up-regulated in ccRCCs. ** p < 0.01; scale bar: 100 µm and 50 µm. c The expression of MTA1 in RCC cell lines. Normal renal cell line HEK293T and RCC cell lines A498 and 768-O cell lysates were used in western blotting analysis with MTA1 and GAPDH antibodies. The bands of MTA1 were from Additional file : Fig. 1 and gapdh were from Additional file : Fig. 1

Article Snippet: The human kidney cancer cell line A498 was purchased from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Expressing, Immunohistochemistry, Staining, Western Blot

Journal: Journal of Healthcare Engineering

Article Title: lncRNA MSC-AS1 Aggravates Diabetic Nephropathy by Regulating the miR-325/CCNG1 Axis

doi: 10.1155/2022/2279072

Figure Lengend Snippet: LncRNA MSC-AS1 knockdown alleviates HG-induced proliferation, fibrosis, and inflammation of HRMC cells. (a) The expression of MSC-AS1 in serum samples from DN patients and normal subjects ( n = 30). (b) The expression of MSC-AS1 in HG- and NG-treated HRMC cells. (c) The expression of MSC-AS1 in HG-treated HRMC cells with its siRNA. (d, e) Cell proliferation and apoptosis were detected in the NG, HG, and HG + si-MSC-AS1 groups. (f) Cell fibrosis was assessed by the levels of FN, Col IV, and Col I in the NG, HG, and HG + si-MSC-AS1 groups. (g) The release of TNF- α , IL-6, and IL-1 β was examined in the NG, HG, and HG + si-MSC-AS1 groups. ∗ P < 0.05 ∗∗ P < 0.01.

Article Snippet: Human renal mesangial cell line HRMC (Procell, Wuhan, China) was cultured in a matched dedicated complete medium (CM-H067; Procell) at 37°C with 5% CO 2 .

Techniques: Knockdown, Expressing

Journal: Journal of Healthcare Engineering

Article Title: lncRNA MSC-AS1 Aggravates Diabetic Nephropathy by Regulating the miR-325/CCNG1 Axis

doi: 10.1155/2022/2279072

Figure Lengend Snippet: lncRNA MSC-AS1 functions as a miR-325 sponge. (a) The binding sites between MSC-AS1 and miR-325. (b) The relationship between MSC-AS1 and miR-325 was verified by pull-down assay. (c) MiR-325 expression was detected in HG-treated HRMC cells with MSC-AS1 siRNA and vector. (d) MSC-AS1 expression was measured in HG-treated HRMC cells containing miR-325 mimics or inhibitors. (e) MiR-325 expression was detected in DN patients and normal subjects ( n = 30). (f) A negative correlation between MSC-AS1 and miR-325 expression was found in DN patients ( n = 30). ∗ P < 0.05, ∗∗ P < 0.01.

Article Snippet: Human renal mesangial cell line HRMC (Procell, Wuhan, China) was cultured in a matched dedicated complete medium (CM-H067; Procell) at 37°C with 5% CO 2 .

Techniques: Binding Assay, Pull Down Assay, Expressing, Plasmid Preparation

Journal: Journal of Healthcare Engineering

Article Title: lncRNA MSC-AS1 Aggravates Diabetic Nephropathy by Regulating the miR-325/CCNG1 Axis

doi: 10.1155/2022/2279072

Figure Lengend Snippet: CCNG1 is a direct target of miR-325. (a) The binding sites between miR-325 and CCNG1. (b) The relationship between miR-325 and CCNG1 was confirmed by pull-down assay. (c) CCNG1 expression was measured in HG-treated HRMC cells containing miR-325 mimics or inhibitors. (d) CCNG1 expression was detected in DN patients and normal subjects. (e) A negative correlation between CCNG1 and miR-325 expression was identified in DN patients ( n = 30). (f) A positive correlation between MSC-AS1 and CCNG1 expression was detected in DN patients ( n = 30). (g) CCNG1 expression was assessed in HG-treated HRMC cells with MSC-AS1 siRNA and vector. ∗ P < 0.05, ∗∗ P < 0.01.

Article Snippet: Human renal mesangial cell line HRMC (Procell, Wuhan, China) was cultured in a matched dedicated complete medium (CM-H067; Procell) at 37°C with 5% CO 2 .

Techniques: Binding Assay, Pull Down Assay, Expressing, Plasmid Preparation

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Combined induction with anti-PD-1 and anti-CTLA-4 antibodies provides synergistic antitumor effects in DC-CIK cells in renal carcinoma cell lines

doi:

Figure Lengend Snippet: Flow cytometry analysis of PD-L1 expression in ACHN and 786 cells. Representative data from at least three independent experiments are shown.

Article Snippet: The two human renal carcinoma cell (RCC) lines ACHN and 786 cells were obtained from Nanjing KeyGen Biotech Co, Ltd (Nanjing, China) and cultured in Dulbecco’s modified Eagle’s medium (GIBCO, USA) containing 10% FBS in a humidified atmosphere of 5% CO 2 at 37°C.

Techniques: Flow Cytometry, Expressing